Synonyms | Fab Preparation Kit enables efficient Fab generation from IgG. This kit uses papain, a nonspecific thiol-endopeptidase, immobilized on agarose resin. Immobilized enzyme is advantageous because digestion can be immediately stopped by simply removing the IgG solution from the resin, resulting in a digest that is enzyme-free. Digestion by papain produces 50kDa Fab and Fc fragments. This complete kit makes Fab generation and purification simple, fast and effective. The kit includes spin columns for easy manipulation of the enzyme resin. The prepacked, immobilized Protein A Plus Spin Column binds the Fc fragments and undigested IgG, allowing the Fab fragments to pass through the spin column for maximum recovery. |