膠體金是由氯金酸(HAuCl4)在還原劑如檸檬酸鈉等作用下,聚合成一定大小的金顆粒,并由于靜電作用成為一種穩(wěn)定的膠體狀態(tài),形成帶負(fù)電的疏水膠溶液,由于靜電作用而成為穩(wěn)定的膠體狀態(tài),故稱膠體金。膠體金在弱堿環(huán)境下帶負(fù)電荷,可與蛋白質(zhì)分子的正電荷基團(tuán)形成牢固的結(jié)合,由于這種結(jié)合是靜電結(jié)合,所以不影響蛋白質(zhì)的生物特性。
膠體金標(biāo)記是蛋白質(zhì)等高分子被吸附到膠體金顆粒表面的包被過(guò)程。吸附機(jī)理可能是膠體金顆粒表面負(fù)電荷,與蛋白質(zhì)的正電荷基團(tuán)因靜電吸附而形成牢固結(jié)合。也可以通過(guò)化學(xué)結(jié)合蛋白。
Passive Absorption
Popular and simple
Cannot provide a permanent attachment of the coating
The proteins may lose their properties after being absorbed to the surface
Covalent Coupling
Permanently immobilizes molecules of interest to functionalized gold nanoparticles
More specific and controllable
Minimizing steric hindrance and impact on the tertiary structure of the conjugated protein
免疫金標(biāo)記技術(shù)主要利用金顆粒具有高電子密度的特性,當(dāng)這些標(biāo)記物在相應(yīng)的配體處大量聚集時(shí),肉眼可見(jiàn)紅色或粉紅色斑點(diǎn),因而用于定性或半定量的快速免疫檢測(cè)方法中。
北京百歐泰生物科技有限公司提供金納米顆粒、金納米棒、銀納米顆粒等納米材料產(chǎn)品,并可以提供偶聯(lián)蛋白/抗體/熒光等服務(wù)。
Our Available Gold Nanoparticles Conjugates
Biotin Gold Nanoparticles
Protein A Gold Nanoparticles
Protein G Gold Nanoparticles
Transferrin Gold Nanoparticles
Streptavidin Gold Nanoparticles
Anti-Goat Gold Nanoparticles
Anti-Sheep Gold Nanoparticles
Anti-Human Gold Nanoparticles
Anti-Mouse Gold Nanoparticles
Anti-Rabbit Gold Nanoparticles
Anti-Rat Gold Nanoparticles
產(chǎn)品特點(diǎn)
Stable
Well Characterized
Customer can select buffer
Customer can select gold nanoparticle type, size
Quick turnaround
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You can place an order or Inquiry through the following methods, and we will contact you ASAP:
QQ 499854788; 82458988
Email [email protected]
Tel 400-669-8850