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抗體產(chǎn)品
- 一抗
  - --|--|流式抗體
  - --|--|單克隆抗體
  - --|--|重組兔單抗
  - --|--|多克隆抗體
  - --|--|小分子抗原抗體
  - --|--|抗體對
  - --|--|標簽抗體
  - --|--|修飾性抗體
  - --|--|其他抗體
- 二抗
- 試劑盒
  - --|--|標記試劑盒
  - --|--|其他試劑盒
  - --|--|ELISA試劑盒
  - --|--|標記染料
蛋白產(chǎn)品
生物材料
- 金屬納米材料
  - --|--|金納米球
  - --|--|金納米棒
  - --|--|銀納米球
  - --|--|金納米海膽
  - --|--|碳納米顆粒
  - --|--|特殊納米顆粒
- 熒光微球
  - --|--|紅色熒光微球
  - --|--|橙色熒光微球
  - --|--|綠色熒光微球
  - --|--|藍色熒光微球
  - --|--|AIE熒光微球
  - --|--|其他熒光微球
- 磁性微球
  - --|--|PS磁性微球
  - --|--|SiO2磁性微球
  - --|--|Fe3O4磁性微球
  - --|--|功能化磁性微球
  - --|--|Magnetic Particles Conjugates
  - --|--|磁性微球試劑盒
  - --|--|其他磁性微球
- 單分散微球
  - --|--|PS微球/聚苯乙烯-100%純度
  - --|--|Silica微球/ 二氧化硅-100%純度
  - --|--|PMMA微球/聚甲基丙烯酸甲酯-100%純度
  - --|--|PET微球/聚對苯二甲酸乙二醇酯-模版法
  - --|--|PVA微球/聚乙烯醇-模版法
  - --|--|PVC微球/聚氯乙烯-100%純度/模版法
  - --|--|DVB微球/二乙烯基苯
  - --|--|PP微球/聚丙烯-模版法
  - --|--|PE微球/聚乙烯-模版法
  - --|--|GMA微球/甲基丙烯酸環(huán)氧丙脂-100%純度
  - --|--|PLA微球/聚乳酸-100%純度
  - --|--|PLGA微球/聚乙丙交酯-100%純度
  - --|--|PLLA微球/聚左旋乳酸-100%純度
  - --|--|PDLA微球/聚右旋乳酸-100%純度
  - --|--|PCL微球/聚己內(nèi)酯-100%純度
  - --|--|PTFE微球/聚四氟乙烯-100%純度
  - --|--|PBS微球聚丁二酸丁二醇酯-100%純度
  - --|--|Polydopamine微球聚多巴胺-100%純度
  - --|--|各向異性janus粒子
  - --|--|Sodium Alginate微球/海藻酸鈉
  - --|--|Agarose微球/瓊脂糖
  - --|--|Polymethylsilsesquioxane微球/聚甲基倍半硅氧烷
  - --|--|PBAT微球/聚己二酸-對苯二甲酸丁二醇酯
- 染色微球
  - --|--|黃色染色微球
  - --|--|黑色染色微球
  - --|--|紅色染色微球
  - --|--|綠色染色微球
  - --|--|紫色染色微球
  - --|--|藍色染色微球
- 其他材料
  - --|--|上轉(zhuǎn)換納米顆粒
  - --|--|量子點
  - --|--|色譜填料
  - --|--|纖維素微球
  - --|--|介孔材料
熱銷產(chǎn)品
- 猴痘相關(guān)產(chǎn)品
- 新冠相關(guān)產(chǎn)品
- 外泌體相關(guān)產(chǎn)品
- 常用試劑
  - --|--|轉(zhuǎn)染試劑
  - --|--|ELISA-FCM試劑
  - --|--|WB試劑
  - --|--|IHC-ICC-IF試劑
  - --|--|載體材料

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馬胰島素酶免試劑盒

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Citations

Cat No	BIOK2006
Conjugate
Type	胰島素含量測定
Source	N/A
Size	96T
Application	ELISA
Format	Liquid
Species	Equine
Storage	Store at 2-8°C upon receipt. Remove any unused antibodycoated strips from the microplate, return them to the foil pouch and re-seal. Once opened, the strips may be stored at 2-8°C for up to one month.
Synonyms	Equine insulin elisa kit
Purification
Note	All reagents and micro-plate strips should be equilibrated to room temperature prior to use. A standard curve must be performed with each assay. It is recommended that all standards and samples should be run in duplicate.Research Use Only. Not for Use in Diagnostic Procedures.
MolecularWeight
Description	Preparation of reagents A. 1×Wash buffer. Prepare 1×Wash buffer by mixing the 10×Wash buffer (20 ml) with 180 ml of distilled water or deionized water. The 1×Wash buffer may be stored at 2-8°C for up to one month. B. 1×Detection antibody solution. Prepare 1×Detection antibody solution by dilution of the 100×Detection antibody solution in Assay buffer, mix well. 70 μl of the 1×Detection antibody solution is required per well. Preparation of samples If a sample has a greater concentration of insulin than the highest standard, the sample should be diluted with 0 μIU/ml insulin standard solution and the assay should be repeated. Assay procedure 1. Add 70 μl of 1x Detection antibody solution to each well. 2. Add 20 μl of standard or sample to its respective well. 3. Cover the plate with a plate sealer. Incubate at room temperature for 1 hour, shaking the plate at 600 rpm on a horizontal microplate shaker. (*Alternative incubation step in the absence of shaker: gently tap the plate frame for a few seconds to ensure thorough mixing, incubate at room temperature for 2 hours.) 4. Discard well contents and remove any remaining solution by inverting and tapping the plate on a clean paper towel. Add 300 μl of 1× Wash buffer to each well. Incubate at room temperature for 30 seconds. 5. Discard the 1× Wash buffer and tap the plate on a clean paper towel to remove residual wash buffer. Repeat the wash step for a total 4 washes. 6. Add 100 μl of Substrate solution to each well, incubate at room temperature for 15 minutes. Protect from light. 7. Add100 μl of Stop solution to each well, gently tap the plate frame for a few seconds to ensure thorough mixing. Measure absorbance of each well at 450 nm immediately. Calculation 1. Subtract the absorbance of the blank from that of standards and samples. 2. Generate a standard curve by plotting the absorbance obtained (y-axis) against insulin concentrations (x-axis) . The best fit line can be generated with any curve-fitting software by regression analysis. Log-log curvefitting is recommended for data analysis. 3. Determine insulin concentration of samples from standard curve. 4. Conversion factor: 1 μIU/ml = 0.0348 ng/ml.
Background	This assay is a sandwich ELISA based on two monoclonal antibodies against separate antigenic determinants on the insulin molecule.

BIOK2006 Equine insulin immunoassay kit.pdf

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馬胰島素酶免試劑盒