Cat No | BSB-021 |
Conjugate | |
Type | 封閉用血清 |
Source | Mouse |
Size | 5ml |
Application | IHC, IF |
Format | Liquid |
Storage | Store at -20 ℃, and avoid repeated freezing and thawing after reconstitution. |
Synonyms | 小鼠血清封閉液;正常血清;動物非免疫血清(小鼠);Normal mouse serum |
Purification | |
Note | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
MolecularWeight | |
Description | 封閉血清適用于免疫組化或者免疫熒光實驗中樣本的封閉.可以封閉待檢樣本中與蛋白質非特異性結合的位點, 另外還可以封閉樣品中內源性的 Fc 片段結合位點, 阻斷抗體與樣品中的 Fc 受體結合, 從而降低背景, 減少假陽性.在選擇封閉血清時, 要注意封閉用血清中不能含有目標蛋白, 且與一抗來源不同, 可用與二抗相同來源的封閉血清.使用方法 免疫組化: 用5-10%正常小鼠血清封閉 ( 1: 10-1: 20倍稀釋) , 室溫孵育5-10分鐘.清除血清, 勿洗, 滴加適當比例稀釋的一抗或一抗工作液, 37℃或室溫孵育4℃過夜. 細胞涂片: 用5-10%正常小鼠血清封閉 ( 1: 10-1: 20倍稀釋) , 使用 PBS 稀釋 ( 0.01mol/L pH7.4) , 少量點滴: 漠過細胞涂片即可. |
Background | Whole serum is obtained from non-immunized (preimmune) healthy mouses. The serum is clarified through a 0.45 micron low protein binding filter. |